Composite

Part:BBa_K3237017:Design

Designed by: Aubrey Demchuk, Kristi Turton   Group: iGEM19_Lethbridge   (2019-10-11)


His-mRFP1-Proinsulin with furin cut sites codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1783
    Illegal XbaI site found at 1771
    Illegal XbaI site found at 1813
    Illegal SpeI site found at 1807
    Illegal PstI site found at 1321
    Illegal PstI site found at 1789
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1783
    Illegal NheI site found at 1335
    Illegal SpeI site found at 1807
    Illegal PstI site found at 1321
    Illegal PstI site found at 1789
    Illegal NotI site found at 1819
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1783
    Illegal BamHI site found at 1801
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1783
    Illegal XbaI site found at 1771
    Illegal XbaI site found at 1813
    Illegal SpeI site found at 1807
    Illegal PstI site found at 1321
    Illegal PstI site found at 1789
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1783
    Illegal XbaI site found at 1771
    Illegal XbaI site found at 1813
    Illegal SpeI site found at 1807
    Illegal PstI site found at 1321
    Illegal PstI site found at 1789
    Illegal AgeI site found at 881
    Illegal AgeI site found at 993
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Extra furin cut site and linker additions were added to provide further processing. The mRFP1 was added as a part of our expression standardization strategy and the his tag was added for potential purification purposes.



Source

This part is an improved part from Tsinhua 2014. The mRFp1 sequence is from the registry (BBa_E1010. The part was synthesized by idt.


References